Characterization of reconstituted partially purified glycerophosphate acyltransferase from Escherichia coli
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چکیده
منابع مشابه
Purification and characterization of glycerophosphate acyltransferase from rat liver mitochondria.
Glycerophosphate acyltransferase (GAT) catalyzes the conversion of sn-glycerol 3-phosphate to lysophosphatidic acid (LPA), the first and committed step of triacylglycerol and phospholipid synthesis. In spite of the important regulatory roles GAT may play in this biosynthetic pathway, little information is available on the structure, biochemical properties, and regulation of GAT from eukaryotic ...
متن کاملBiosynthesis of phosphatidyl glycerophosphate in Escherichia coli.
An enzyme (L-glycerol 3-phosphate: CMP phosphatidyltransferase) catalyzing the synthesis of phosphatidyl glycerophosphate from CDP-diglyceride and L-glycerol 3-phosphate has been rendered soluble by treatment of the particulate, membrane-containing fraction of E. coli with Triton X-100 and has been partially purified. The enzyme, devoid of phosphatidyl glycerophosphatase activity, is specific f...
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Overproduction of the sn-glycerol-3-phosphate acyltransferase in Escherichia coli leads to incorporation of this integral membrane protein into ordered tubular arrays within the cell. Freeze-fracture-etch shadowing was performed on suspensions of partially purified tubules and whole bacteria. This procedure revealed the presence of ridges and grooves defining a set of long-pitch left-handed hel...
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Desulfovibrio africanus ferredoxin III is a monomeric protein (molecular mass of 6585 Da) that contains one [3Fe-4S]1+/0 and one [4Fe-4S]2+/1+ cluster when isolated aerobically. The amino acid sequence consists of 61 amino acids, including seven cysteine residues that are all involved in co-ordination to the clusters. In order to isolate larger quantities of D. africanus ferredoxin III, we have...
متن کاملMembranous structure of purified Escherichia coli lipopolysaccharide.
The ultrastructure of the purified and lyophilized endotoxin from Escherichia coli O111 was observed by ultrathin sectioning. Onion-like globular membrane structures were observed in addition to rod-like and ribbon-like structures, indicating the existence of a globular membrane structure even in the dried state.
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
سال: 1982
ISSN: 0005-2760
DOI: 10.1016/0005-2760(82)90317-4